Nanomaterials (Nov 2020)
Effect of Remineralized Collagen on Dentin Bond Strength through Calcium Phosphate Ion Clusters or Metastable Calcium Phosphate Solution
Abstract
This study aimed to investigate whether dentin remineralization and micro-tensile bond strength increase when using calcium phosphate ion clusters (CPICs) or metastable Ca-P. After being etched, each dentin specimen was designated into four groups and treated with the appropriate solution for 1 min: 100% ethanol, 2 and 1 mg/mL of CPICs, and metastable Ca-P. The specimens were then prepared for scanning electron microscopy (SEM), transmission electron microscropy (TEM) imaging, a matrix metalloproteinases inhibition assay, and the micro-tensile bond strength test. To compare among the groups, one-way analysis of variance was performed. In the SEM imaging, with a rising concentration of CPICs, the degree of remineralization of dentin increased significantly. The metastable Ca-P treated specimens showed a similar level of remineralization as the 1 mg/mL CPICs treated specimens. The TEM imaging also revealed that dentin remineralization occurs in a CPICs concentration-dependent manner between the demineralized dentin and the resin layer. Furthermore, the results of micro-tensile bond strength showed the same trend as the results confirmed by SEM and TEM. We demonstrated that a 1 min pretreatment of CPICs or metastable Ca-P in etched dentin collagen fibril can achieve biomimetic remineralization and increase micro-tensile bond strength.
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