CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Toon Swings; David C. Marciano; Benu Atri; Rachel E. Bosserman; Chen Wang; Marlies Leysen; Camille Bonte; Thomas Schalck; Ian Furey; Bram Van den Bergh; Natalie Verstraeten; Peter J. Christie; Christophe Herman; Olivier Lichtarge; Jan Michiels

doi:10.1038/s41467-018-04651-5

Nature Communications (Jun 2018)

CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Toon Swings,
David C. Marciano,
Benu Atri,
Rachel E. Bosserman,
Chen Wang,
Marlies Leysen,
Camille Bonte,
Thomas Schalck,
Ian Furey,
Bram Van den Bergh,
Natalie Verstraeten,
Peter J. Christie,
Christophe Herman,
Olivier Lichtarge,
Jan Michiels

Affiliations

Toon Swings: Centre of Microbial and Plant Genetics, KU Leuven - University of Leuven
David C. Marciano: Department of Molecular and Human Genetics, Baylor College of Medicine
Benu Atri: Quantitative and Computational Biosciences, Baylor College of Medicine
Rachel E. Bosserman: Department of Microbiology and Molecular Genetics, McGovern Medical School
Chen Wang: Department of Molecular and Human Genetics, Baylor College of Medicine
Marlies Leysen: Centre of Microbial and Plant Genetics, KU Leuven - University of Leuven
Camille Bonte: Centre of Microbial and Plant Genetics, KU Leuven - University of Leuven
Thomas Schalck: Centre of Microbial and Plant Genetics, KU Leuven - University of Leuven
Ian Furey: Department of Pharmacology, Baylor College of Medicine
Bram Van den Bergh: Centre of Microbial and Plant Genetics, KU Leuven - University of Leuven
Natalie Verstraeten: Centre of Microbial and Plant Genetics, KU Leuven - University of Leuven
Peter J. Christie: Department of Microbiology and Molecular Genetics, McGovern Medical School
Christophe Herman: Department of Molecular and Human Genetics, Baylor College of Medicine
Olivier Lichtarge: Department of Molecular and Human Genetics, Baylor College of Medicine
Jan Michiels: Centre of Microbial and Plant Genetics, KU Leuven - University of Leuven

DOI: https://doi.org/10.1038/s41467-018-04651-5
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

Read online

Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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