Comprehensive micro-scaled proteome and phosphoproteome characterization of archived retrospective cancer repositories

Corinna Friedrich; Simon Schallenberg; Marieluise Kirchner; Matthias Ziehm; Sylvia Niquet; Mohamed Haji; Christin Beier; Jens Neudecker; Frederick Klauschen; Philipp Mertins

doi:10.1038/s41467-021-23855-w

Nature Communications (Jun 2021)

Comprehensive micro-scaled proteome and phosphoproteome characterization of archived retrospective cancer repositories

Corinna Friedrich,
Simon Schallenberg,
Marieluise Kirchner,
Matthias Ziehm,
Sylvia Niquet,
Mohamed Haji,
Christin Beier,
Jens Neudecker,
Frederick Klauschen,
Philipp Mertins

Affiliations

Corinna Friedrich: German Cancer Consortium (DKTK), partner site Berlin
Simon Schallenberg: Institute of Pathology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
Marieluise Kirchner: Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Proteomics Platform
Matthias Ziehm: Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Proteomics Platform
Sylvia Niquet: Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Proteomics Platform
Mohamed Haji: Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Proteomics Platform
Christin Beier: Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Proteomics Platform
Jens Neudecker: Department of Surgery - Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin
Frederick Klauschen: German Cancer Consortium (DKTK), partner site Berlin
Philipp Mertins: German Cancer Consortium (DKTK), partner site Berlin

DOI: https://doi.org/10.1038/s41467-021-23855-w
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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Formalin-fixed, paraffin-embedded (FFPE) specimens are an attractive resource for retrospective clinical proteomics studies. Here, the authors benchmark and optimize proteomics workflows for FFPE sample analysis and provide guidelines on which methods to use for different samples and applications.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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