Endophytic Bacillus megaterium BM18-2 mutated for cadmium accumulation and improving plant growth in Hybrid Pennisetum

Abstract

Hybrid Pennisetum (Pennisetum americanum × P. purpureum Schumach L.) is a tall and rapidly growing perennial C4 bunch grass. It has been considered as a promising plant for phytoremediation of heavy metal-contaminated soil due to its high biomass, high resistance to environmental stress, pests and diseases. Heavy metal bioavailability level is the most important parameter for measurement of the phytoremediation efficiency. Endophytic bacteria were used to further enhance phytoremediation of heavy metals through bioaccumulation or bioabsorption process. In the present study, the endophytic Bacillus megaterium strain ‘BM18’ isolated from hybrid Pennisetum was screened under 10-70 μM cadmium (Cd) stress for Cd-resistant mutant colonies. And one such mutant colony‘BM18-2’ was obtained from the screen. Comparably, ‘BM18-2’ was more Cd-tolerant and had higher Cd removal ability than the original strain‘BM18’. The amount of IAA and ammonia production, and phosphate solubilization were 1.09, 1.23 and 1.24 times in ‘BM18-2’ than those of ‘BM18’, respectively. Full genome sequencing of these two strains revealed 6 different genes: BM18GM000901, BM18GM005669 and BM18GM005870 encoding heavy metal efflux pumps, BM18GM003487 and BM18GM005818 encoding transcriptional regulators for metal stress biosensor and BM18GM001335 encoding a replication protein. Inoculation with ‘BM18-2’ or ‘BM18’ both significantly reduced the toxic effect of Cd on hybrid Pennisetum, while the effect of ‘BM18-2’ on plant growth promotion in the presence of Cd was significantly better that of ‘BM18’. Therefore, the mutated strain ‘BM18-2’ could be used as a potential agent for Cd bioremediation, improving growth and Cd absorption of hybrid Pennisetum in Cd contaminated soil. Keywords: Bacillus megaterium, Bioremediation, PGP, Hybrid Pennisetum, Endophytes, Cadmium