Biodegradation of Wastes using Partially Purified Cellulose-Enzyme from Gut of Oryctes rhinoceros larvae from Rafia Palms in Itokin, Lagos State, Nigeria

Abstract

Conversion of urban wastes to utilizable sugars is very important as a vital step towards reduction of environmental pollution. Consequently, this work was aimed at obtaining cellulase from the gut of Oryctes rhinoceros larvae from raffia palms in Itokin, Lagos State, Nigeria to evaluate its degradability potential on different plant leaves and selected plastic bottles. Cellulase from Oryctes rhinoceros larvae was partially purified by a combination of precipitation with ammonium sulphate and fractionation with gel filtration on Biogel P- 100. The cellulase degradability potential was examined on tree leaves from cashew, banana, maize, lemon, cassava, pawpaw, mango, almond and palm respectively, while the plastics were from bottles of teem,pepsi, eva, mirinda, cascade, still, coke, devana and nestle respectively. Data obtained show that the enzyme- cellulase has a specific activity of 2.38 Unit/mg with 32% yield with higher degradability potential on samples from plant origin than the materials from industrial wastes.

Keywords

• Oryctes;
• rhinoceros;
• cellulose;
• degradability