DIFFERENTIATED PRE-ADIPOCYTES ACT AS A DRIVING FORCE FOR PROSTATE CANCER PROLIFERATION, MIGRATION AND EPITHELIAL-MESENCHYMAL TRANSITION

Abstract

Obesity is a popular topic due to both its mortality and morbidity rates and related diseases such as cardiovascular diseases, diabetes and cancer. Cancer development and progression relate to many factors one of which is dysfunctional adipocytes found in the tumor microenvironment. However, underlying molecular mechanisms of the obesity-cancer link have not been fully understood. In the current study, condition media (CM) obtained from differentiated pre-adipocytes was used to set an indirect co-culture system with the prostate cancer cell line. Firstly, adipogenesis of 3T3F44-2A was checked by oil red o staining and expressions of specific genes. CM of differentiated 3T3F44-2A was applied on prostate cancer cells (PC3). Cell viability, migration capacity and related gene expression levels of the cells were evaluated. 20% CM increased cell viability after 48h. The administration also induced proliferation, migration and epithelial-mesenchymal transition (EMT)-related gene expressions. The results presented the roles of adipocytes found in the tumor microenvironment and this could allow new therapeutic developments. As a new perspective to evaluate the obesity-cancer link, our model experiment may also be useful for other cancer types.

Keywords

• pre-adipocyte
• obesity
• pre-adipocyte
• prostate cancer cells
• adipogenesis
• condition media
• tumor microenvironment
• emt