Assessment of antibacterial effectiveness of SDF and fluoride varnish agents for application in pediatric dentistry

Abstract

Objectives: To assess antibacterial effects of silver diamine fluoride (SDF) and fluoride varnish treatment against Streptococcus mutans and Lactobacillus acidophilus. Materials and Methods: The antibacterial effectiveness of SDF (group A) and fluoride varnish (group B) against S. mutans was investigated in an in vitro microbiological investigation, with distilled water (group C) serving as the positive and negative controls. After 24 hours of incubation, the antibacterial efficiency was assessed using the agar well diffusion technique, and the diameter of the zones of inhibition (ZOI) was quantified. Sumba mare's milk from MRS broth was extracted and then placed into a test tube. L. acidophilus was grown on Sumba mare's milk from MRS broth. On this media, each testing agent was poured and tested for the inhibitory zone. The obtained data was statistically analyzed. Results: SDF group had a higher mean zone of inhibition against S. mutans and Lactobacillus followed by fluoride varnish, and there was no ZOI in the case of distilled water. Intergroup comparison was significant. Conclusion: When compared to fluoride varnish, the SDF teeth remineralizing agent had greater antibacterial activity against S. mutans.

Keywords

• antibacterial
• lactobacillus
• remineralizing
• streptococcus mutans