Polarization modulated spectroscopic ellipsometry-based surface plasmon resonance biosensor for E. coli K12 detection

Abstract

Abstract In this work, we report on the application of the polarization modulated spectroscopic ellipsometry-based surface plasmon resonance method for sensitive detection of microorganisms in Kretschmann configuration. So far, rotating analyzer and single wavelength polarization modulation methods have widely been investigated for phase sensitive surface plasmon resonance measurement. In this study, a much simpler optical setup relying on fast electro-optic phase modulator crystals is introduced for bacteria detection. A beta barium borate crystal connected to a function generator is adapted for generating phase shifts in the millisecond regime to extract the ellipsometric angles ( $$\Psi$$ Ψ and $$\Delta$$ Δ ) under the surface plasmon resonance condition. For detection, the gold surface was functionalized with anti-Escherichia coli antibodies, and E. coli K12 was attached to them. We show that polarization modulated spectroscopic ellipsometry achieves a refractive index resolution in the order of $$10^{-5}$$ 10 - 5 RIU, and a limit of detection of $$10^{2}$$ 10 2 CFU/mL for E. coli K12 which is compatible with other surface plasmon resonance based phase sensitive methods with more complex detection concepts. As a follow-up step, an optical model can be developed to enhance this biosensor’s performance, and applications for sorting and detecting other biological targets will be investigated.

Keywords

• Spectroscopic ellipsometry
• Surface plasmon resonance
• Electro-optic polarization modulation
• E. coli bacteria