Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts

Benedict M. Long; Wei Yih Hee; Robert E. Sharwood; Benjamin D. Rae; Sarah Kaines; Yi-Leen Lim; Nghiem D. Nguyen; Baxter Massey; Soumi Bala; Susanne von Caemmerer; Murray R. Badger; G. Dean Price

doi:10.1038/s41467-018-06044-0

Nature Communications (Sep 2018)

Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts

Benedict M. Long,
Wei Yih Hee,
Robert E. Sharwood,
Benjamin D. Rae,
Sarah Kaines,
Yi-Leen Lim,
Nghiem D. Nguyen,
Baxter Massey,
Soumi Bala,
Susanne von Caemmerer,
Murray R. Badger,
G. Dean Price

Affiliations

Benedict M. Long: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University
Wei Yih Hee: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University
Robert E. Sharwood: Australian Research Council Centre of Excellence for Translational Photosynthesis, Research School of Biology, The Australian National University
Benjamin D. Rae: Australian Research Council Centre of Excellence for Translational Photosynthesis, Research School of Biology, The Australian National University
Sarah Kaines: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University
Yi-Leen Lim: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University
Nghiem D. Nguyen: Australian Research Council Centre of Excellence for Translational Photosynthesis, Research School of Biology, The Australian National University
Baxter Massey: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University
Soumi Bala: Australian Research Council Centre of Excellence for Translational Photosynthesis, Research School of Biology, The Australian National University
Susanne von Caemmerer: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University
Murray R. Badger: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University
G. Dean Price: Realizing Increased Photosynthetic Efficiency (RIPE), The Australian National University

DOI: https://doi.org/10.1038/s41467-018-06044-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 14

Abstract

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Previous efforts to assemble Rubisco within a cyanobacterial carboxysome-derived protein shell in plant chloroplasts to concentrate CO2 have been unsuccessful. Here, Long et al. produce carboxysomes in tobacco chloroplasts that encapsulate the introduced Rubisco and enable autotrophic growth at elevated CO2.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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