Determination of the activity of some antioxidant enzymes during the germination periods of cereals and legumes

Abstract

As a result of the exchange of substances in a living organism, “products” “free radicals” and peroxide compounds of organic and inorganic substances are formed. It is known that free radicals damage cells. Enzymes of the body's antioxidant defense system, superoxide dismutase, catalase, glutathione reductase, glutathione peroxidases, and toxic products of lipid peroxide oxidation turn free radicals into harmless substances. In this work, the activity of one of such enzymes - peroxidase, catalase was studied. The activity of catalase enzyme during the germination periods of cereal and leguminous plants is different, the highest activity of the catalase enzyme during the germination periods of cereal plants was determined on the 5th day of the germination period: in wheat – 5,644 mg H2O2/g, in barley – 4,08 mg H2O2/g, in oats – 5,134 mg H2O2/g. In leguminous plants, the highest activity was determined on the 3rd day of the germination period: it was 11,832 mg H2O2/g in soybean, and 8,126 mg H2O2/g in mash. The highest activity of peroxidase enzyme is on the first day of wheat and is 1,158 µmol/min.g mass. In leguminous plants, the highest activity of the peroxidase enzyme is on the 9th day of the soybean germination period, and its activity is 0,687 µmol/min.g. constituted the mass.