Easy-to-Use InDel Markers for Genetic Mapping between Col-0 and L<i>er</i>-0 Accessions of <i>Arabidopsis thaliana</i>

Abstract

Map-based gene cloning has played a key role in many genetic studies using the model plant, Arabidopsis thaliana. In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on Arabidopsis mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and Ler-0 accessions that can be detected in standard agarose gel electrophoresis.

Keywords

• InDel markers
• SSLP
• chromosome mapping
• <i>Arabidopsis thaliana</i>
• mutants