Protocol for generating dormant human brain metastatic breast cancer spheroids in vitro

Raghu Vamsi Kondapaneni; Sumiran Kumar Gurung; Lalita A. Shevde; Shreyas S. Rao

STAR Protocols (Jun 2024)

Protocol for generating dormant human brain metastatic breast cancer spheroids in vitro

Raghu Vamsi Kondapaneni,
Sumiran Kumar Gurung,
Lalita A. Shevde,
Shreyas S. Rao

Affiliations

Raghu Vamsi Kondapaneni: Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL, USA; Corresponding author
Sumiran Kumar Gurung: Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL, USA
Lalita A. Shevde: Department of Pathology, O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA
Shreyas S. Rao: Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL, USA; Corresponding author

Journal volume & issue: Vol. 5, no. 2
p. 102962

Abstract

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Summary: Here, we present a protocol to generate dormant brain metastatic breast cancer (BMBC) spheroids utilizing hyaluronic acid (HA) hydrogels. We describe the steps for construction of spheroids from human BMBC cell lines MDA-MB-231Br and BT474Br3, HA hydrogel preparation, and spheroid plating on HA hydrogels and in suspension culture. We then detail the impact of HA hydrogel on the dormant phenotype of spheroids by measuring spheroid cross-sectional area, cell numbers, and EdU staining.For complete details on the use and execution of this protocol, please refer to Kondapaneni et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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