Electronic Journal of Plant Breeding (Jul 2010)
Efficiency and effectiveness of physical and chemical mutagens and their combination in inducing chlorophyll mutations in M2 generation of Lablab [Lablab purpureus (l.) Sweet var. Typicus]
Abstract
A comparative study of the frequency and spectrum of chlorophyll mutations induced by gamma rays (150, 200, 250, 300 and350 Gy), sodium azide (0.5, 1.0, 1.5 and 2.0 mM) and combination treatments of gamma rays and sodium azide (100 Gy + 1.5mM, 150 Gy + 1.5 mM, 200 Gy + 1.0 mM, 250 Gy + 1.0 mM and 300 Gy + 0.5 mM) in M2 generationA was made in thelablab var. CO 9. Four different types of chlorophyll mutants viz., albina, viridis, chlorina and xantha were identified in thetreated population and mutation frequency was calculated on plant population basis. Frequency of chlorina mutants washighest followed by albina, xantha and viridis. Combination treatments in general proved to be very effective followed bysodium azide and gamma rays in inducing maximum frequency of chlorophyll mutations. Although the chlorophyll mutationsdo not have any economic value due to their lethal nature, such a study could be useful in identifying the threshold dose of amutagen that would increase the genetic variability and number of economically useful mutants in the segregating generations.
