A multi-omics approach to visualize early neuronal differentiation from hESCs in 4D

Athina Samara; Mari Spildrejorde; Ankush Sharma; Martin Falck; Magnus Leithaug; Stefania Modafferi; Pål Marius Bjørnstad; Ganesh Acharya; Kristina Gervin; Robert Lyle; Ragnhild Eskeland

iScience (Nov 2022)

A multi-omics approach to visualize early neuronal differentiation from hESCs in 4D

Athina Samara,
Mari Spildrejorde,
Ankush Sharma,
Martin Falck,
Magnus Leithaug,
Stefania Modafferi,
Pål Marius Bjørnstad,
Ganesh Acharya,
Kristina Gervin,
Robert Lyle,
Ragnhild Eskeland

Affiliations

Athina Samara: Division of Clinical Paediatrics, Department of Women’s and Children’s Health, Karolinska Institutet, Solna, Sweden; Astrid Lindgren Children′s Hospital Karolinska University Hospital, Stockholm, Sweden; Corresponding author
Mari Spildrejorde: PharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway; Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway
Ankush Sharma: Department of Informatics, University of Oslo, Oslo, Norway; Department of Molecular Medicine, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway; Department of Biosciences, University of Oslo, Oslo, Norway
Martin Falck: PharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway; Department of Biosciences, University of Oslo, Oslo, Norway
Magnus Leithaug: Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway
Stefania Modafferi: Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway
Pål Marius Bjørnstad: Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway
Ganesh Acharya: Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Alfred Nobels Allé 8, SE-14152 Stockholm, Sweden; Center for Fetal Medicine, Karolinska University Hospital Huddinge, SE-14186 Stockholm, Sweden
Kristina Gervin: PharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway; Pharmacoepidemiology and Drug Safety Research Group, Department of Pharmacy, School of Pharmacy, University of Oslo, Oslo, Norway; Division of Clinical Neuroscience, Department of Research and Innovation, Oslo University Hospital, Oslo, Norway
Robert Lyle: PharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway; Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway; Centre for Fertility and Health, Norwegian Institute of Public Health, Oslo, Norway; Corresponding author
Ragnhild Eskeland: PharmaTox Strategic Research Initiative, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway; Department of Molecular Medicine, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway; Corresponding author

Journal volume & issue: Vol. 25, no. 11
p. 105279

Abstract

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Summary: Neuronal differentiation of pluripotent stem cells is an established method to study physiology, disease, and medication safety. However, the sequence of events in human neuronal differentiation and the ability of in vitro models to recapitulate early brain development are poorly understood. We developed a protocol optimized for the study of early human brain development and neuropharmacological applications. We comprehensively characterized gene expression and epigenetic profiles at four timepoints, because the cells differentiate from embryonic stem cells towards a heterogeneous population of progenitors, immature and mature neurons bearing telencephalic signatures. A multi-omics roadmap of neuronal differentiation, combined with searchable interactive gene analysis tools, allows for extensive exploration of early neuronal development and the effect of medications.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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