Pathogens (Oct 2023)

The Green Synthesis of Silver Nanoparticles from <i>Avena fatua</i> Extract: Antifungal Activity against <i>Fusarium oxysporum</i> f.sp. <i>lycopersici</i>

  • Ahmad Kaleem Qureshi,
  • Umar Farooq,
  • Qaiser Shakeel,
  • Sajjad Ali,
  • Sarfraz Ashiq,
  • Sohail Shahzad,
  • Muhammad Tariq,
  • Mahmoud F. Seleiman,
  • Aftab Jamal,
  • Muhammad Farhan Saeed,
  • Barbara Manachini

DOI
https://doi.org/10.3390/pathogens12101247
Journal volume & issue
Vol. 12, no. 10
p. 1247

Abstract

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Using plant extracts as eco-friendly reducing and stabilizing agents for the synthesis of nanoparticles has gained significant attention in recent years. The current study explores the green synthesis of silver nanoparticles (AgNPs) using the Avena fatua extract and evaluates their antifungal activity against Fusarium oxysporum f.sp. lycopersici (Fol), a fungal plant pathogen. A green and sustainable approach was adopted to synthesize silver nanoparticles before these nanoparticles were employed for anti-fungal activity. The primary indication that AgNPs had formed was performed using UV-vis spectroscopy, where a strong peak at 425 nm indicated the effective formation of these nanoparticles. The indication of important functional groups acting as reducing and stabilizing agents was conducted using the FTIR study. Additionally, morphological studies were executed via SEM and AFM, which assisted with more effectively analyzing AgNPs. Crystalline behavior and size were estimated using powder XRD, and it was found that AgNPs were highly crystalline, and their size ranged from 5 to 25 nm. Synthesized AgNPs exhibited significant antifungal activity against Fol at a concentration of 40 ppm. Furthermore, the inhibitory index confirmed a positive correlation between increasing AgNPs concentration and exposure duration. This study suggests that the combined phytochemical mycotoxic effect of the plant extract and the smaller size of synthesized AgNPs were responsible for the highest penetrating power to inhibit Fol growth. Moreover, this study highlights the potential of using plant extracts as reducing and capping agents for the green synthesis of AgNPs with antifungal properties. The study concludes that A. fatua extract can synthesize antifungal AgNPs as a sustainable approach with robust antifungal efficacy against Fol, underscoring their promising potential for integration into plant protection strategies.

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