In vitro propagation of endemic species Hedysarum chaiyrakanicum (Tuva Republic, Russia) and its widespread congener, H. gmelini (Fabaceae)

Abstract

A rare species Hedysarum chaiyrakanicum and a highly polymorphic species H. gmelini were first introduced into in vitro culture. It was shown that MS supplemented by 5 µM BAP is optimal medium for micropropagation of H. gmelini from 90-1 and 92-1 subpopulations, medium with 1 µM BAP is optimal for micropropagation of H. chaiyrakanicum from 45-2 subpopulation, and 10 µM BAP – for H. chaiyrakanicum from 45-1 subpopulation. Seedlings obtained from the seeds of H. gmelinii collected from the subpopulation no. 88 demonstrated a higher tendency to callus formation using BAP. It was also found that in vitro culture of H. chaiyrakanikum was characterized by a higher reproduction rate then H. gmelinii in vitro culture.