Nature Communications (Sep 2021)

Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis

  • Fredarla S. Miller,
  • Kathryn K. Crone,
  • Matthew R. Jensen,
  • Sudipta Shaw,
  • William R. Harcombe,
  • Mikael H. Elias,
  • Michael F. Freeman

DOI
https://doi.org/10.1038/s41467-021-25575-7
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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Borosins are ribosomally encoded and posttranslationally modified peptide (RiPP) natural products featuring amide-backbone α-N-methylation. Here, the authors report the discovery and characterization of type IV borosin ‘split’ pathways encoding distinct, separate α-N-methyltransferases and precursor peptide substrates.