Effect of Essential Oils of Syzygium aromaticum and Melaleuca alternifolia on Isolates of Aspergillus sp.

Abstract

Essential oils have antimicrobial substances, lower cost and the lower resistance of microorganism. This study aimed to evaluate the inhibition of mycelial growth of Aspergillus flavus and A. niger with essential oils of Syzygium aromaticum and Melaleuca alternifolia. For this, aliquots (0.5, 5, 10 and 15μL) of essential oils from S. aromaticum and M. alternifolia were distributed on the surface of the culture medium Potato Dextrose Agar (PDA) with Drigaslsky. In the control treatment were used only plates containing PDA plus chloramphenicol (1%). After 2 hours, a disc (8mm diameter) of the isolated mycelium, with 10 days of age on PDA, was peaked to the center of the plates, these plates were sealed and incubated at 282°C, in the dark. The essential oil of S. aromaticum inhibited the mycelial growth of A. flavus and A. niger, in all aliquots. When using the essential oil of M. alternifolia decreased the mycelial growth of A. flavus in all aliquots, however, showed a low efficiency in control of A. niger. It was concluded that the essential oil S. aromaticum is effective against A. flavus and A. niger, which can be used in control against these microorganisms, and M. alternifolia not show satisfactory results in relation to reducing the growth of pathogens evaluated.

Keywords

• onychomycosis
• alternative therapy
• antifungal