Standardisation of microencapsulation protocol for chilled preservation of Malabari buck spermatozoa

Abstract

The present study was conducted to standardise the protocol for microencapsulation of buck spermatozoa for its chilled preservation. Semen ejaculates collected from Malabari bucks of age two to three years, weighing 42 to 46 Kg and maintained under uniform managemental conditions were utilised. Sodium alginate of concentrations 0.5, 0.75 and 1.5 per cent with varying gauge sized hypodermic needles of 18G, 20G and 23G were used. The distance between the tip of the needle and the upper meniscus of BaCl2 solution was adjusted to varied distance like ≥ 7 cm, 3.5 to 5 cm, ≤ 2cm. This resulted in capsule sizes of 1 mm, 2 mm, 3 mm, 4 mm, 4.5 mm, 6 mm and 7 mm. Different shape of capsules like globular, plate like, irregular, tear-drop and oval were obtained and the capsule stability varied from 35 to 75 per cent. Capsule parameters were assessed at 0 h, 24 h, 48 h, 72 h and 96 h of chilled preservation in EYC extender at ratio of (1:2). The results of the present study cited that 1.5 per cent sodium alginate concentration, with 23G sized hypodermic needle and distance of 3.5 cm were ideal to acquire uniform spherical shaped stable capsule of size 1.90 ± 0.03 and stability of 75 per cent Keywords: Microencapsulation, chilled preservation, buck spermatozoa, Malabari