npj Microgravity (Mar 2021)
Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium
- Jennifer Barrila,
- Shameema F. Sarker,
- Nicole Hansmeier,
- Shanshan Yang,
- Kristina Buss,
- Natalia Briones,
- Jin Park,
- Richard R. Davis,
- Rebecca J. Forsyth,
- C. Mark Ott,
- Kevin Sato,
- Cristine Kosnik,
- Anthony Yang,
- Cheryl Shimoda,
- Nicole Rayl,
- Diana Ly,
- Aaron Landenberger,
- Stephanie D. Wilson,
- Naoko Yamazaki,
- Jason Steel,
- Camila Montano,
- Rolf U. Halden,
- Tom Cannon,
- Sarah L. Castro-Wallace,
- Cheryl A. Nickerson
Affiliations
- Jennifer Barrila
- Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University
- Shameema F. Sarker
- Biodesign Center for Infectious Diseases and Vaccinology, Arizona State University
- Nicole Hansmeier
- Swette Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University
- Shanshan Yang
- Bioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State University
- Kristina Buss
- Bioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State University
- Natalia Briones
- Bioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State University
- Jin Park
- Bioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State University
- Richard R. Davis
- Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University
- Rebecca J. Forsyth
- Biodesign Center for Infectious Diseases and Vaccinology, Arizona State University
- C. Mark Ott
- Biomedical Research and Environmental Sciences Division, NASA Johnson Space Center
- Kevin Sato
- NASA Ames Research Center
- Cristine Kosnik
- Tissue Genesis, Inc
- Anthony Yang
- Tissue Genesis, Inc
- Cheryl Shimoda
- Tissue Genesis, Inc
- Nicole Rayl
- NASA Ames Research Center
- Diana Ly
- NASA Ames Research Center
- Aaron Landenberger
- DoD Space Test Program
- Stephanie D. Wilson
- Astronaut Office, NASA Johnson Space Center
- Naoko Yamazaki
- Space Biomedical Research Office, Human Space Technology and Astronauts Department, Japan Aerospace Exploration Agency (JAXA)
- Jason Steel
- Bioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State University
- Camila Montano
- Bioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State University
- Rolf U. Halden
- Biodesign Center for Environmental Health Engineering, School of Sustainable Engineering and the Built Environment, Arizona State University
- Tom Cannon
- Tissue Genesis, Inc
- Sarah L. Castro-Wallace
- Biomedical Research and Environmental Sciences Division, NASA Johnson Space Center
- Cheryl A. Nickerson
- Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University
- DOI
- https://doi.org/10.1038/s41526-021-00136-w
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 10
Abstract
Abstract Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE study flown aboard Space Shuttle mission STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection with Salmonella Typhimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this was the first in-flight infection and dual RNA-seq analysis using human cells.
