<i>Lactoplantibacillus plantarum</i> KG Lysates Inhibit the Internalization of <i>Staphylococcus aureus</i> by Human Keratinocytes through the Induction of Human Beta-Defensin 3

Abstract

Human β-defensin 3 (hBD3) is an antimicrobial peptide and a chemoattractant molecule expressed in human keratinocytes. Lysates prepared from Lactoplantibacillus plantarum KG (KG lysates), a fermented lactobacillus strain with Ginseng extracts, significantly induced hBD3 in human keratinocytes. hBD3 induced by KG lysates affected the internalization of Staphylococcus aureus by human keratinocytes. The intracellular pathway for the induction of hBD3 by KG lysates was examined using inhibitors. Briefly, KG lysates stimulated the late signaling pathway including JAK1/2, which might be induced by the EGFR- or IFN-γ-mediated pathway, and affected hBD3 induction. Through this study, we confirmed that KG lysates have the ability to control bacterial infections, especially S. aureus, in human keratinocytes. Thus, KG lysates can be provided in various formulations for the development of food and pharmaceutical materials to control bacterial infection.

Keywords

• probiotics lysates
• antimicrobial peptide
• HaCaT cells
• cellular infection
• EGFR
• JAK-STAT pathway