Frontiers in Microbiology (Jan 2019)

Detection and Characterization of ESBL-Producing Escherichia coli From Humans and Poultry in Ghana

  • Linda Falgenhauer,
  • Linda Falgenhauer,
  • Can Imirzalioglu,
  • Can Imirzalioglu,
  • Kwabena Oppong,
  • Charity Wiafe Akenten,
  • Benedikt Hogan,
  • Benedikt Hogan,
  • Ralf Krumkamp,
  • Ralf Krumkamp,
  • Sven Poppert,
  • Sven Poppert,
  • Vinzent Levermann,
  • Vinzent Levermann,
  • Oliver Schwengers,
  • Nimako Sarpong,
  • Ellis Owusu-Dabo,
  • Jürgen May,
  • Jürgen May,
  • Daniel Eibach

DOI
https://doi.org/10.3389/fmicb.2018.03358
Journal volume & issue
Vol. 9

Abstract

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Introduction: The increasing incidence of infections caused by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in sub-Saharan Africa is of serious concern. Studies from countries with a highly industrialized poultry industry suggest the poultry production-food-consumer chain as a potential transmission route. In Africa, integrated studies at this human–animal interface are still missing.Aim: To determine the molecular epidemiology of ESBL-producing E. coli from the intestinal tract of humans and poultry in rural Ghana.Methods: During a 6-month period, fecal samples from all children admitted to the Agogo Hospital (Ghana) and broilers at eight poultry farms located within the hospital catchment area were collected. After screening on selective ESBL agar, whole genome sequencing (WGS) was performed on all ESBL isolates. The genomes were analyzed using multilocus sequence typing (MLST), ESBL genotyping and genome-based phylogenetic analyses.Results: Of 140 broilers and 54 children, 41 (29%) and 33 (61%) harbored ESBL E. coli, respectively, with prevalences on farms ranging between 0 and 85%. No predominant sequence type (ST) was detected among humans. ST10 was most prevalent among broilers (n = 31, 69%). The ESBL gene blaCTX-M-15 was predominant among broilers (n = 43, 96%) and humans (n = 32, 97%). Whole-genome-based phylogenetic analysis revealed three very closely related broiler/human isolate clusters (10% of ESBL isolates) with chromosomal and plasmid-mediated ESBL genes.Conclusion: The findings demonstrate a high frequency of intestinal ESBL-producing E. coli in rural Ghana. Considering that animal and human samples are independent specimens from the same geographic location, the number of closely related ESBL isolates circulating across these two reservoirs is substantial. Hence, poultry farms or meat products might be an important source for ESBL-producing bacteria in rural Ghana leading to difficult-to-treat infections in humans.

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