Alʹmanah Kliničeskoj Mediciny (Feb 2016)
BENIGN EPITHELIAL NEOPLASIA ASSOCIATED WITH BETA-HUMAN PAPILLOMA VIRUS
Abstract
Aim: To study an association between acrochordon and human papilloma virus (HPV) using quantitative analysis of viral desoxyribonucleic acid (DNA); to detect different phenotypes of beta-HPV. Materials and methods: We examined 52 patients (22 immuno-suppressed patients and 30 immunocompetent subjects) in the Dermatovenereology and Dermato-Oncology Department and Chronic Dialysis and Kidney Transplantation Department of the Moscow Regional Research and Clinical Institute (MONIKI). Control group included 49 healthy donors. Burr biopsy samples (micro-samples) of acrochordon and intact skin (apper arm) were collected in sterile conditions. After sample procession and DNA isolation using DNK-sorb-C kit (Central Research Institute for Epidemiology – CRIE), polymerase chain reaction for HPV was performed with real-time fluorescent hybridization detection. For DNA amplification and detection we used RotorGene 3000 analyzer (Corbett Research, Australia). In the beta-HPV assay, recombinant plasmids were used as positive controls and control human beta-globin gene fragments (CRIE). 4 oligo-nucleotide systems (group-specific primers and probes) were used for the detection of beta-HPV DNA. Results: Preliminary data indicated that acrochordons of open and covered skin regions were common in renal transplant recipients. Beta-HPV DNA was more frequent in acrochordons and intact skin (64% and 54%) of renal transplant recipients compared to healthy donors (47%). 57% of renal transplant recipients demonstrated mixed infection in acrochordons. Conclusion: HPV DNA was frequently detected in acrochordons and intact skin of renal transplant recipients. In immunocompetent patients prevalence of HPV DNA in acrochordons was significantly higher compared to intact skin.
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