Frontiers in Plant Science (Aug 2024)
Arabidopsis TIC236 contributes to proplastid development and chloroplast biogenesis during embryogenesis
- Mei Liu,
- Mei Liu,
- Mei Liu,
- Mei Liu,
- Mei Liu,
- Lifen Chen,
- Lifen Chen,
- Lifen Chen,
- Lifen Chen,
- Lifen Chen,
- Shijie Gu,
- Shijie Gu,
- Shijie Gu,
- Shijie Gu,
- Shijie Gu,
- Aiwei Zhang,
- Aiwei Zhang,
- Aiwei Zhang,
- Aiwei Zhang,
- Aiwei Zhang,
- Mengjuan Tong,
- Mengjuan Tong,
- Mengjuan Tong,
- Mengjuan Tong,
- Mengjuan Tong,
- Shuailei Wang,
- Shuailei Wang,
- Shuailei Wang,
- Shuailei Wang,
- Shuailei Wang,
- Juntao Wang,
- Juntao Wang,
- Juntao Wang,
- Juntao Wang,
- Juntao Wang,
- Yirui Zhu,
- Yirui Zhu,
- Yirui Zhu,
- Yirui Zhu,
- Yirui Zhu,
- Jingsheng Zhang,
- Jingsheng Zhang,
- Jingsheng Zhang,
- Jingsheng Zhang,
- Jingsheng Zhang,
- Yu Sun,
- Yu Sun,
- Yu Sun,
- Yu Sun,
- Yu Sun,
- Yi Guo,
- Yi Guo,
- Yi Guo,
- Yi Guo,
- Yi Guo,
- Rui Li,
- Rui Li,
- Rui Li,
- Rui Li,
- Rui Li
Affiliations
- Mei Liu
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Mei Liu
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Mei Liu
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Mei Liu
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Mei Liu
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Lifen Chen
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Lifen Chen
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Lifen Chen
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Lifen Chen
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Lifen Chen
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Shijie Gu
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Shijie Gu
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Shijie Gu
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Shijie Gu
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Shijie Gu
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Aiwei Zhang
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Aiwei Zhang
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Aiwei Zhang
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Aiwei Zhang
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Aiwei Zhang
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Mengjuan Tong
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Mengjuan Tong
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Mengjuan Tong
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Mengjuan Tong
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Mengjuan Tong
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Shuailei Wang
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Shuailei Wang
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Shuailei Wang
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Shuailei Wang
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Shuailei Wang
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Juntao Wang
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Juntao Wang
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Juntao Wang
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Juntao Wang
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Juntao Wang
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Yirui Zhu
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Yirui Zhu
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Yirui Zhu
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Yirui Zhu
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Yirui Zhu
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Jingsheng Zhang
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Jingsheng Zhang
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Jingsheng Zhang
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Jingsheng Zhang
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Jingsheng Zhang
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Yu Sun
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Yu Sun
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Yu Sun
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Yu Sun
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Yu Sun
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Yi Guo
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Yi Guo
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Yi Guo
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Yi Guo
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Yi Guo
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- Rui Li
- Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Normal University, Shijiazhuang, China
- Rui Li
- Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Rui Li
- Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Normal University, Shijiazhuang, China
- Rui Li
- Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, China
- Rui Li
- College of Life Sciences, Hebei Normal University, Shijiazhuang, China
- DOI
- https://doi.org/10.3389/fpls.2024.1424994
- Journal volume & issue
-
Vol. 15
Abstract
Plastids are essential, semi-autonomous organelles in plants that carry out a multitude of functions during development. Plastids existing in different subtypes are derived from proplastids progenitors and interconvert in response to environmental and growth cues. Most efforts focus on the differentiation from proplastid to other forms. However, the studies of proplastid development are insufficient and whether proplastid biogenesis affects plant growth is yet to be determined. Arabidopsis TIC236, a translocon component at the inner membrane of the chloroplast envelope, is critical for importing chloroplast-targeted preproteins and chloroplast division. In this study, we uncovered the fundamental influence of proplastid biogenesis on embryo development by exploring the function of TIC236 during embryogenesis. Widespread and strong expression of TIC236 was observed in leaves and embryos. The null mutant tic236 had an embryo-lethal phenotype, with cell division in the mutant embryos delayed starting at the octant stage and arrested at the globular stage. Transmission electron microscopy revealed enlarged proplastids with an aberrant inner structure at the dermatogen and globular stages that ultimately did not differentiate into chloroplasts. Additionally, the fluorescence signal distribution patterns of tic236 embryos carrying the pDR5rev::3xVENUS-N7, pPIN1::PIN1-GFP, pWOX5::GFP, and pSCR::H2B-YFP reporter systems were altered. Together, we provide genetic evidence supporting proplastid biogenesis plays a vital role in embryo development and TIC236 is identified as an indispensable player, ensuring normal proplastid development.
Keywords
