Prevalence of Antimicrobial Resistance and Clonal Relationship in ESBL/AmpC-Producing <i>Proteus mirabilis</i> Isolated from Meat Products and Community-Acquired Urinary Tract Infection (UTI-CA) in Southern Brazil

Matheus Silva Sanches; Luana Carvalho Silva; Caroline Rodrigues da Silva; Victor Hugo Montini; Bruno Henrique Dias de Oliva; Gustavo Henrique Migliorini Guidone; Mara Corrêa Lelles Nogueira; Maísa Fabiana Menck-Costa; Renata Katsuko Takayama Kobayashi; Eliana Carolina Vespero; Sergio Paulo Dejato Rocha

doi:10.3390/antibiotics12020370

Antibiotics (Feb 2023)

Prevalence of Antimicrobial Resistance and Clonal Relationship in ESBL/AmpC-Producing <i>Proteus mirabilis</i> Isolated from Meat Products and Community-Acquired Urinary Tract Infection (UTI-CA) in Southern Brazil

Matheus Silva Sanches,
Luana Carvalho Silva,
Caroline Rodrigues da Silva,
Victor Hugo Montini,
Bruno Henrique Dias de Oliva,
Gustavo Henrique Migliorini Guidone,
Mara Corrêa Lelles Nogueira,
Maísa Fabiana Menck-Costa,
Renata Katsuko Takayama Kobayashi,
Eliana Carolina Vespero,
Sergio Paulo Dejato Rocha

Affiliations

Matheus Silva Sanches: Laboratory of Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Luana Carvalho Silva: Laboratory of Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Caroline Rodrigues da Silva: Microorganism Research Center, Health Sciences Center, Department of Dermatological, Infectious and Parasitic Diseases, Medical School of São José do Rio Preto, São José do Rio Preto P.O. Box 15.090, Brazil
Victor Hugo Montini: Laboratory of Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Bruno Henrique Dias de Oliva: Laboratory of Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Gustavo Henrique Migliorini Guidone: Laboratory of Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Mara Corrêa Lelles Nogueira: Microorganism Research Center, Health Sciences Center, Department of Dermatological, Infectious and Parasitic Diseases, Medical School of São José do Rio Preto, São José do Rio Preto P.O. Box 15.090, Brazil
Maísa Fabiana Menck-Costa: Laboratory of Basic and Applied Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Renata Katsuko Takayama Kobayashi: Laboratory of Basic and Applied Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Eliana Carolina Vespero: Department of Pathology, Health Sciences Center, Clinical and Toxicological Analysis, University Hospital of Londrina, State University of Londrina, Londrina P.O. Box 10.011, Brazil
Sergio Paulo Dejato Rocha: Laboratory of Bacteriology, Center of Biological Sciences, Department of Microbiology, State University of Londrina, Londrina P.O. Box 10.011, Brazil

DOI: https://doi.org/10.3390/antibiotics12020370
Journal volume & issue: Vol. 12, no. 2
p. 370

Abstract

Read online

The present study aimed to evaluate the prevalence of antimicrobial resistance and clonal relationships in Proteus mirabilis isolated from chicken meat, beef, pork, and community-acquired urinary tract infections (UTI-CA). Chicken meat isolates showed the highest multidrug resistance (MDR), followed by those from pork and UTI-CA, whereas beef had relatively few MDR strains. All sources had strains that carried blaCTX-M-65, whereas blaCTX-M-2 and blaCMY-2 were only detected in chicken meat and UTI-CA isolates. This indicates that chicken meat should be considered an important risk factor for the spread of P. mirabilis carrying ESBL and AmpC. Furthermore, ESBL/AmpC producing strains were resistant to a greater number of antimicrobials and possessed more resistance genes than non-producing strains. In addition, the antimicrobial resistance genes qnrD, aac(6′)-Ib-cr, sul1, sul2, fosA3, cmlA, and floR were also found. Molecular typing showed a genetic similarity between chicken meat and UTI-CA isolates, including some strains with 100% similarity, indicating that chicken can be a source of P. mirabilis causing UTI-CA. It was concluded that meat, especially chicken meat, can be an important source of dissemination of multidrug-resistant P. mirabilis in the community.

Published in Antibiotics

ISSN: 2079-6382 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antibiotics

About the journal

Abstract

Keywords