Nature Communications (Oct 2017)

HDX reveals the conformational dynamics of DNA sequence specific VDR co-activator interactions

  • Jie Zheng,
  • Mi Ra Chang,
  • Ryan E. Stites,
  • Yong Wang,
  • John B. Bruning,
  • Bruce D. Pascal,
  • Scott J. Novick,
  • Ruben D. Garcia-Ordonez,
  • Keith R. Stayrook,
  • Michael J. Chalmers,
  • Jeffrey A. Dodge,
  • Patrick R. Griffin

DOI
https://doi.org/10.1038/s41467-017-00978-7
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 13

Abstract

Read online

The vitamin D receptor/retinoid X receptor-α heterodimer (VDRRXRα) regulates bone mineralization. Here the authors employ hydrogen/deuterium exchange (HDX) mass spectrometry to study the conformational dynamics of VDRRXRα and give mechanistic insights into how VDRRXRα controls the transcriptional activity of specific genes.