Newly Isolated Virulent Salmophages for Biocontrol of Multidrug-Resistant <i>Salmonella</i> in Ready-to-Eat Plant-Based Food

Michał Wójcicki; Olga Świder; Paulina Średnicka; Dziyana Shymialevich; Tomasz Ilczuk; Łukasz Koperski; Hanna Cieślak; Barbara Sokołowska; Edyta Juszczuk-Kubiak

doi:10.3390/ijms241210134

International Journal of Molecular Sciences (Jun 2023)

Newly Isolated Virulent Salmophages for Biocontrol of Multidrug-Resistant <i>Salmonella</i> in Ready-to-Eat Plant-Based Food

Michał Wójcicki,
Olga Świder,
Paulina Średnicka,
Dziyana Shymialevich,
Tomasz Ilczuk,
Łukasz Koperski,
Hanna Cieślak,
Barbara Sokołowska,
Edyta Juszczuk-Kubiak

Affiliations

Michał Wójcicki: Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland
Olga Świder: Department of Food Safety and Chemical Analysis, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland
Paulina Średnicka: Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland
Dziyana Shymialevich: Culture Collection of Industrial Microorganisms—Microbiological Resources Center, Department of Microbiology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland
Tomasz Ilczuk: Department of Pathology, Medical University of Warsaw, Pawińskiego 7 Str., 02-106 Warsaw, Poland
Łukasz Koperski: Department of Pathology, Medical University of Warsaw, Pawińskiego 7 Str., 02-106 Warsaw, Poland
Hanna Cieślak: Culture Collection of Industrial Microorganisms—Microbiological Resources Center, Department of Microbiology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland
Barbara Sokołowska: Department of Microbiology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland
Edyta Juszczuk-Kubiak: Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, Rakowiecka 36 Str., 02-532 Warsaw, Poland

DOI: https://doi.org/10.3390/ijms241210134
Journal volume & issue: Vol. 24, no. 12
p. 10134

Abstract

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Due to irrational antibiotic stewardship, an increase in the incidence of multidrug resistance of bacteria has been observed recently. Therefore, the search for new therapeutic methods for pathogen infection treatment seems to be necessary. One of the possibilities is the utilization of bacteriophages (phages)—the natural enemies of bacteria. Thus, this study is aimed at the genomic and functional characterization of two newly isolated phages targeting MDR Salmonella enterica strains and their efficacy in salmonellosis biocontrol in raw carrot–apple juice. The Salmonella phage vB_Sen-IAFB3829 (Salmonella phage strain KKP 3829) and Salmonella phage vB_Sen-IAFB3830 (Salmonella phage strain KKP 3830) were isolated against S. I (6,8:l,-:1,7) strain KKP 1762 and S. Typhimurium strain KKP 3080 host strains, respectively. Based on the transmission electron microscopy (TEM) and whole-genome sequencing (WGS) analyses, the viruses were identified as members of tailed bacteriophages from the Caudoviricetes class. Genome sequencing revealed that these phages have linear double-stranded DNA and sizes of 58,992 bp (vB_Sen-IAFB3829) and 50,514 bp (vB_Sen-IAFB3830). Phages retained their activity in a wide range of temperatures (from −20 °C to 60 °C) and active acidity values (pH from 3 to 11). The exposure of phages to UV radiation significantly decreased their activity in proportion to the exposure time. The application of phages to the food matrices significantly reduced the level of Salmonella contamination compared to the control. Genome analysis showed that both phages do not encode virulence or toxin genes and can be classified as virulent bacteriophages. Virulent characteristics and no possible pathogen factors make examined phages feasible to be potential candidates for food biocontrol.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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