Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction

Laercio Uemura; Rossana Baggio Simeoni; Paulo André Bispo Machado Júnior; Gustavo Gavazzoni Blume; Luize Kremer Gamba; Murilo Sgarbossa Tonial; Paulo Ricardo Baggio Simeoni; Victoria Stadler Tasca Ribeiro; Rodrigo Silvestre; Katherine Athayde Teixeira de Carvalho; Marcelo Henrique Napimoga; Júlio Cesar Francisco; Luiz Cesar Guarita-Souza

doi:10.3390/jfb13020059

Journal of Functional Biomaterials (May 2022)

Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction

Laercio Uemura,
Rossana Baggio Simeoni,
Paulo André Bispo Machado Júnior,
Gustavo Gavazzoni Blume,
Luize Kremer Gamba,
Murilo Sgarbossa Tonial,
Paulo Ricardo Baggio Simeoni,
Victoria Stadler Tasca Ribeiro,
Rodrigo Silvestre,
Katherine Athayde Teixeira de Carvalho,
Marcelo Henrique Napimoga,
Júlio Cesar Francisco,
Luiz Cesar Guarita-Souza

Affiliations

Laercio Uemura: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Rossana Baggio Simeoni: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Paulo André Bispo Machado Júnior: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Gustavo Gavazzoni Blume: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Luize Kremer Gamba: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Murilo Sgarbossa Tonial: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Paulo Ricardo Baggio Simeoni: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Victoria Stadler Tasca Ribeiro: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Rodrigo Silvestre: Instituto de Radiologia (InRad), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05508-070, Brazil
Katherine Athayde Teixeira de Carvalho: Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, 1632 Silva Jardim Avenue, Curitiba 80240-020, Brazil
Marcelo Henrique Napimoga: Institute and Research Center São Leopoldo Mandic, São Leopoldo Mandic, Faculty–SLMANDIC, Campinas, São Paulo 13045-775, Brazil
Júlio Cesar Francisco: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil
Luiz Cesar Guarita-Souza: Experimental Laboratory of Institute of Biological and Health Sciences, Pontifícia Universidade Católica do Paraná (PUCPR), 1555 Imaculada Conceição Street, Curitiba 80215-901, Brazil

DOI: https://doi.org/10.3390/jfb13020059
Journal volume & issue: Vol. 13, no. 2
p. 59

Abstract

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To investigate the effect of transplantation of stem cells from the bone marrow mononuclear cells (BMMC) associated with 15d-PGJ2-loaded nanoparticles in a rat model of chronic MI. Chronic myocardial infarction (MI) was induced by the ligation of the left anterior descending artery in 40 male Wistar rats. After surgery, we transplanted bone marrow associated with 15d-PGJ2-loaded nanoparticle by intramyocardial injection (106 cells/per injection) seven days post-MI. Myocardial infarction was confirmed by echocardiography, and histological analyses of infarct morphology, gap junctions, and angiogenesis were obtained. Our results from immunohistochemical analyses demonstrated the presence of angiogenesis identified in the transplanted region and that there was significant expression of connexin-43 gap junctions, showing a more effective electrical and mechanical integration of the host myocardium. This study suggests that the application of nanoparticle technology in the prevention and treatment of MI is an emerging field and can be a strategy for cardiac repair.

Published in Journal of Functional Biomaterials

ISSN: 2079-4983 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology; Medicine: Medicine (General)
Website: http://www.mdpi.com/journal/jfb/

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