A protocol for acquiring high-quality single-cell multi-omics data from human peripheral blood

Abstract

Summary: Single-cell analysis of human peripheral blood cells provides insights into innate and adaptive immune systems. However, robust protocols are essential to ensuring single-cell sequencing data quality and cell viability. Here, we present a protocol for acquiring high-quality single-cell multi-omics data from human peripheral blood mononuclear cells (PBMCs). We describe steps for collecting human blood followed by single-cell sequencing, whole-genome sequencing, and metabolome and proteome analysis of PBMCs using modified multi-omics sample processing. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Clinical Protocol
• Genomics
• Biotechnology and bioengineering