Nature Communications (Jul 2021)
BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination
- Rania Ghouil,
- Simona Miron,
- Lieke Koornneef,
- Jasper Veerman,
- Maarten W. Paul,
- Marie-Hélène Le Du,
- Esther Sleddens-Linkels,
- Sari E. van Rossum-Fikkert,
- Yvette van Loon,
- Natalia Felipe-Medina,
- Alberto M. Pendas,
- Alex Maas,
- Jeroen Essers,
- Pierre Legrand,
- Willy M. Baarends,
- Roland Kanaar,
- Sophie Zinn-Justin,
- Alex N. Zelensky
Affiliations
- Rania Ghouil
- Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Uni Paris-Sud, Uni Paris-Saclay
- Simona Miron
- Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Uni Paris-Sud, Uni Paris-Saclay
- Lieke Koornneef
- Department of Developmental Biology, Oncode Institute, Erasmus University Medical Center
- Jasper Veerman
- Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center
- Maarten W. Paul
- Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center
- Marie-Hélène Le Du
- Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Uni Paris-Sud, Uni Paris-Saclay
- Esther Sleddens-Linkels
- Department of Developmental Biology, Erasmus University Medical Center
- Sari E. van Rossum-Fikkert
- Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center
- Yvette van Loon
- Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center
- Natalia Felipe-Medina
- Molecular Mechanisms Program, Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca)
- Alberto M. Pendas
- Molecular Mechanisms Program, Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca)
- Alex Maas
- Department of Cell Biology, Erasmus University Medical Center
- Jeroen Essers
- Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center
- Pierre Legrand
- Synchrotron SOLEIL, L’Orme des Merisiers
- Willy M. Baarends
- Department of Developmental Biology, Erasmus University Medical Center
- Roland Kanaar
- Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center
- Sophie Zinn-Justin
- Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Uni Paris-Sud, Uni Paris-Saclay
- Alex N. Zelensky
- Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center
- DOI
- https://doi.org/10.1038/s41467-021-24871-6
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 16
Abstract
BRCA2 and its interactor HSF2BP are required for meiotic recombination. Here, the authors define the interaction structurally, revealing that a repeat in BRCA2 binds two HSF2BP units, increasing the affinity. This region is, however, not essential for mouse meiosis.
