Тонкие химические технологии (Apr 2012)
Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers
Abstract
An indirect approach for the determination of enantiomeric purity of chiral α-(benzyl)-carboxyethyl- and γ-methyl-substituted monomers of polyamide nucleic acids mimics (PANAM) is described. The test consist in the synthesis of C-terminal diastereomers with methyl ester of L- or DL-isoleucine for each PANAM monomer and estimation of the relative content (abundance ratio) of main and minor diastereomers by means of reverse phase HPLC and 1H-NMR spectroscopy.