المجلة العراقية للاستزراع المائي (Dec 2020)

Micropropagation of sugarcane Saccharum officinarum Plant via Organogensis callus in vitro

  • Haleemah J. AL-Arady,
  • Abbas M. Jasim,
  • Muayad F. Abbas

DOI
https://doi.org/10.58629/ijaq.v17i2.73
Journal volume & issue
Vol. 17, no. 2

Abstract

Read online

Propagation of sugarcane Saccharum officinarum (L) Plant via in vitro by callus production through the culture of shoot tips on MS medium supplemented by 2,4- D 3 mg/L. Organogenic callus were produced by culturing callus on MS medium contained BA and kinetin at 0.5 mg/L for both of them. Incubation of culture was at 25 c and photoperiod at 16 hr and darkness for 8 hrs. Adventitious shoots were cultured on rooting medium contained MS salts at half concentration and NAA 0.5 mg/L , BA 0.5 mg/L and incubated for 8 weeks. Then plantlets were produced and successfully acclimatized.

Keywords