iScience (Jul 2021)
Comparative analysis of clonal evolution among patients with right- and left-sided colon and rectal cancer
- Santasree Banerjee,
- Xianxiang Zhang,
- Shan Kuang,
- Jigang Wang,
- Lei Li,
- Guangyi Fan,
- Yonglun Luo,
- Shuai Sun,
- Peng Han,
- Qingyao Wu,
- Shujian Yang,
- Xiaobin Ji,
- Yong Li,
- Li Deng,
- Xiaofen Tian,
- Zhiwei Wang,
- Yue Zhang,
- Kui Wu,
- Shida Zhu,
- Lars Bolund,
- Huanming Yang,
- Xun Xu,
- Junnian Liu,
- Yun Lu,
- Xin Liu
Affiliations
- Santasree Banerjee
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Xianxiang Zhang
- Department of Gastroenterology, General Surgery Center, The Affiliated Hospital of Qingdao University, Qingdao 266555, China
- Shan Kuang
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Jigang Wang
- Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao 266555, China
- Lei Li
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 101408, China
- Guangyi Fan
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Yonglun Luo
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark
- Shuai Sun
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Peng Han
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Qingyao Wu
- Department of Gastroenterology, General Surgery Center, The Affiliated Hospital of Qingdao University, Qingdao 266555, China
- Shujian Yang
- Department of Gastroenterology, General Surgery Center, The Affiliated Hospital of Qingdao University, Qingdao 266555, China
- Xiaobin Ji
- Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao 266555, China
- Yong Li
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Li Deng
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen 518083, China
- Xiaofen Tian
- BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; MGI, BGI-Shenzhen, Shenzhen 518083, China
- Zhiwei Wang
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Yue Zhang
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Kui Wu
- BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Shida Zhu
- BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
- Lars Bolund
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; Department of Biomedicine, Aarhus University, Aarhus 8000, Denmark; Lars Bolund Institute of Regenerative Medicine, BGI-Qingdao, BGI-Shenzhen, Qingdao, China
- Huanming Yang
- BGI-Shenzhen, Shenzhen 518083, China; James D. Watson Institute of Genome Sciences, Hangzhou 310058, Zhejiang, China
- Xun Xu
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Genome Read and Write, BGI-Shenzhen, Shenzhen, Guangdong, China
- Junnian Liu
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; Corresponding author
- Yun Lu
- Department of Gastroenterology, General Surgery Center, The Affiliated Hospital of Qingdao University, Qingdao 266555, China; Shandong Key Laboratory of Digital Medicine and Computer Assisted Surgery, Qingdao University, Qingdao, China; Corresponding author
- Xin Liu
- BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China; BGI-Shenzhen, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China; Corresponding author
- Journal volume & issue
-
Vol. 24,
no. 7
p. 102718
Abstract
Summary: Tumor multiregion sequencing reveals intratumor heterogeneity (ITH) and clonal evolution playing a key role in tumor progression and metastases. Large-scale high-depth multiregional sequencing of colorectal cancer, comparative analysis among patients with right-sided colon cancer (RCC), left-sided colon cancer (LCC), and rectal cancer (RC), as well as the study of lymph node metastasis (LN) with extranodal tumor deposits (ENTDs) from evolutionary perspective remain weakly explored. Here, we recruited 68 patients with RCC (18), LCC (20), and RC (30). We performed high-depth whole-exome sequencing of 206 tumor regions including 176 primary tumors, 19 LN, and 11 ENTD samples. Our results showed ITH with a Darwinian pattern of evolution and the evolution pattern of LCC and RC was more complex and divergent than RCC. Genetic and evolutionary evidences found that both LN and ENTD originated from different clones. Moreover, ENTD was a distinct entity from LN and evolved later.
