Substitution of the Methionine Axial Ligand of the T1 Copper for the Fungal-like Phenylalanine Ligand (M298F) Causes Local Structural Perturbations that Lead to Thermal Instability and Reduced Catalytic Efficiency of the Small Laccase from Streptomyces coelicolor A3(2)

• Kairit Zovo,
• Hegne Pupart,
• Arie Van Wieren,
• Richard E. Gillilan,
• Qingqiu Huang,
• Sudipta Majumdar,
• Tiit Lukk

Affiliations

Kairit Zovo

Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia

Hegne Pupart

Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia

Arie Van Wieren

Department of Chemistry, Biochemistry, Physics and Engineering, Indiana University of Pennsylvania, Indiana, Pennsylvania, United States

Richard E. Gillilan

MacCHESS (Macromolecular Diffraction Facility at CHESS), Cornell University, Ithaca, New York, United States

Qingqiu Huang

MacCHESS (Macromolecular Diffraction Facility at CHESS), Cornell University, Ithaca, New York, United States

Sudipta Majumdar

Department of Chemistry, Biochemistry, Physics and Engineering, Indiana University of Pennsylvania, Indiana, Pennsylvania, United States

Tiit Lukk

Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia