Substantiation of the choice of the model for the formation of oxidative stress in preclinical studies

Abstract

At the preclinical stage, a comparative assessment of various models of the formation of oxidative stress by exposure to high and low temperatures, ultraviolet irradiation, and the influence of a low-frequency alternating magnetic field was carried out. Exposure to laboratory animals was carried out daily for 21 days. It has been established that the modeling of oxidative stress by exposure to ultraviolet rays on rats allows, by the end of the first week of the experiment, to induce an increase in the intensity of lipid peroxidation processes with the accumulation of lipid peroxidation products by 48-61% and a decrease in the activity of antioxidant components by 31-33% in comparison with the control, which exceeds similar parameters on day 7 in models of hypothermia, hyperthermia and magnetic induction. Cold exposure in rats is accompanied by a more pronounced shift in the antioxidant status towards the prooxidant side by the end of the third week, which is confirmed by an increase in the concentration of lipid peroxidation products by 45-67% and a decrease in the level of components of the antioxidant system by 28-37% relative to the control. In general, the prooxidant effect in vivo of all the studied effects was confirmed, which we recommend as experimental models for the formation of oxidative stress at the preclinical stage, substantiating in the work the possibility of using each effect depending on the purpose of the pharmacological study.