Heat shock protein 90 alpha family class B member 1 (HSP90AB1) gene polymorphism and its effect on milk production traits in friesian holstein cattle

Abstract

Increasing temperature caused by climate change is main contributor of heat stress in dairy cattle. Heat stress has a major impact on the milk production efficiency in dairy cattle. The HSP90AB1 gene is involved in overcoming heat stress response in cattle. The purpose of this study is to find single-nucleotide polymorphism (SNP) of HSP90AB1 gene and its relationship to milk production traits in Friesian Holstein (FH) cattle. In this study, 50 FH blood samples were used. Allele Specific Polymerase Chain Reaction (AS-PCR) method was used to successfully identify three genotypes: CC, CT, and TT. The CT genotype is the most common in the sample population. When compared to the C-allele, the T-allele is more common. Hardy-Weinberg (HW) analysis using Chi-Square method revealed that the population was not equilibrium (P0.05), but the CC genotype had a trend of higher mean milk yield in the first and second lactation. It was determined that the HSP90AB1 gene could be used as molecular marker for FH cattle heat stress response, in order to increase milk production capacity.