Journal of Analytical Science and Technology (Mar 2010)
Determination of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) surfactant with liquid chromatography: Comparative study of evaporative light scattering detector, ultraviolet detector and conductivity detector
Abstract
This work presents comparison of performance of ultraviolet (UV) detector, conductivity detector (CD) and evaporative light scattering detector (ELSD) in terms of quantitative analysis of AOT (sodium bis(2-ethylhexyl)sulfosuccinate) using liquid chromatography. The employed chromatographic condition, including an acetonitrile/water (45:55, v/v) isocratic eluent system, is suitable for the three different detectors, and the figures of merits obtained by building up calibration plots are compared. The sensitivities of the detectors are in the order of ELSD ≈ CD >> UV detector. The linear range for quantification of AOT depends on the type of detector: the lower limits are in the order of UV detector (207 ㎍ mL-1) < CD (310 ㎍ mL-1) << ELSD (930 ㎍ mL-1), while the upper limits are 3720 ㎍ mL-1 for all the detectors (the maximum concentration of injected standard solution). The detection limits are 155 ㎍ mL-1 for ELSD, 78 ㎍ mL-1 for UV detector and 13 ㎍ mL-1 for CD, respectively. The figures of merit for each detector could be a guideline in choosing a detector in quantization of AOT. Furthermore, application of the chromatographic method to two commercial products is demonstrated.
