Frontiers in Microbiology (Aug 2021)
Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer
- Li Zhang,
- Li Zhang,
- Li Zhang,
- Li Huang,
- Li Huang,
- Li Huang,
- Mi Huang,
- Mi Huang,
- Mi Huang,
- Mengying Wang,
- Mengying Wang,
- Mengying Wang,
- Dekang Zhu,
- Dekang Zhu,
- Mingshu Wang,
- Mingshu Wang,
- Mingshu Wang,
- Renyong Jia,
- Renyong Jia,
- Renyong Jia,
- Shun Chen,
- Shun Chen,
- Shun Chen,
- Xinxin Zhao,
- Xinxin Zhao,
- Xinxin Zhao,
- Qiao Yang,
- Qiao Yang,
- Qiao Yang,
- Ying Wu,
- Ying Wu,
- Ying Wu,
- Shaqiu Zhang,
- Shaqiu Zhang,
- Shaqiu Zhang,
- Juan Huang,
- Juan Huang,
- Juan Huang,
- Xumin Ou,
- Xumin Ou,
- Xumin Ou,
- Sai Mao,
- Sai Mao,
- Sai Mao,
- Qun Gao,
- Qun Gao,
- Qun Gao,
- Bin Tian,
- Bin Tian,
- Bin Tian,
- Anchun Cheng,
- Anchun Cheng,
- Anchun Cheng,
- Mafeng Liu,
- Mafeng Liu,
- Mafeng Liu
Affiliations
- Li Zhang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Li Zhang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Li Zhang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Li Huang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Li Huang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Li Huang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Mi Huang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mi Huang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Mi Huang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Mengying Wang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mengying Wang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Mengying Wang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Dekang Zhu
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Dekang Zhu
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Mingshu Wang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mingshu Wang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Mingshu Wang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Renyong Jia
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Renyong Jia
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Renyong Jia
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Shun Chen
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shun Chen
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Shun Chen
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Xinxin Zhao
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Xinxin Zhao
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Xinxin Zhao
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Qiao Yang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Qiao Yang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Qiao Yang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Ying Wu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ying Wu
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Ying Wu
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Shaqiu Zhang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shaqiu Zhang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Shaqiu Zhang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Juan Huang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Juan Huang
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Juan Huang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Xumin Ou
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Xumin Ou
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Xumin Ou
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Sai Mao
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Sai Mao
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Sai Mao
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Qun Gao
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Qun Gao
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Qun Gao
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Bin Tian
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Bin Tian
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Bin Tian
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Anchun Cheng
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Anchun Cheng
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Anchun Cheng
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- Mafeng Liu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mafeng Liu
- Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Mafeng Liu
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China
- DOI
- https://doi.org/10.3389/fmicb.2021.644868
- Journal volume & issue
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Vol. 12
Abstract
Riemerella anatipestifer is a gram-negative bacterium that is the first naturally competent bacterium identified in the family Flavobacteriaceae. However, the determinants that influence the natural transformation and the underlying mechanism remain unknown. In this study, we evaluated the effects of various nutritional factors of the GCB medium [glucose, L-glutamine, vitamin B1, Fe (NO3)3, NaCl, phosphate, and peptone], on the natural transformation of R. anatipestifer ATCC 11845. Among the assayed nutrients, peptone and phosphate affected the natural transformation of R. anatipestifer ATCC 11845, and the transformation frequency was significantly decreased when phosphate or peptone was removed from the GCB medium. When the iron chelator 2,2′-dipyridyl (Dip) was added, the transformation frequency was decreased by approximately 100-fold and restored gradually when Fe (NO3)3 was added, suggesting that the natural transformation of R. anatipestifer ATCC 11845 requires iron. Given the importance of TonB in nutrient transportation, we further identified whether TonB is involved in the natural transformation of R. anatipestifer ATCC 11845. Mutation of tonBA or tonBB, but not tbfA, was shown to inhibit the natural transformation of R. anatipestifer ATCC 11845 in the GCB medium. In parallel, it was shown that the tonBB mutant, but not the tonBA mutant, decreased iron acquisition in the GCB medium. This result suggested that the tonBB mutant affects the natural transformation frequency due to the deficiency of iron utilization.
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