Generation of integration-free Down syndrome and isogenic euploid human induced pluripotent stem cells

Sunita Nehra; Vishi Sharma; Malati Umrani; Nishant Singhal

Stem Cell Research (Mar 2023)

Generation of integration-free Down syndrome and isogenic euploid human induced pluripotent stem cells

Sunita Nehra,
Vishi Sharma,
Malati Umrani,
Nishant Singhal

Affiliations

Sunita Nehra: Stem cell and Neurobiology Lab, National Centre for Cell Science (NCCS), Pune 411007, India
Vishi Sharma: Stem cell and Neurobiology Lab, National Centre for Cell Science (NCCS), Pune 411007, India
Malati Umrani: Stem cell and Neurobiology Lab, National Centre for Cell Science (NCCS), Pune 411007, India
Nishant Singhal: Corresponding author.; Stem cell and Neurobiology Lab, National Centre for Cell Science (NCCS), Pune 411007, India

Journal volume & issue: Vol. 67
p. 103041

Abstract

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A pair of Down syndrome (DS) human iPSCs (hiPSCs) and isogenic euploid hiPSCs generated by using an integration-free Sendai viral vector system showed trisomy 21 (47; XY) and typical (46; XY) karyotype respectively. Pluripotency of both hiPSC lines was confirmed by pluripotency marker expression and three germ layer differentiation potentials.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal