Detection of the SARS-CoV-2 Nucleocaspid Protein (NP) Using Immunohistochemistry

Zeqing Feng; Bo Diao; Rongshuai Wang; Gang Wang; Chenhui Wang; Yingjun Tan; Liang Liu; Changsong Wang; Ying Liu; Yueping Liu; Zilin Yuan; Liang Ren; Yuzhang Wu; Yongwen Chen

doi:10.21769/BioProtoc.5002

Bio-Protocol (Apr 2020)

Detection of the SARS-CoV-2 Nucleocaspid Protein (NP) Using Immunohistochemistry

Zeqing Feng,
Bo Diao,
Rongshuai Wang,
Gang Wang,
Chenhui Wang,
Yingjun Tan,
Liang Liu,
Changsong Wang,
Ying Liu,
Yueping Liu,
Zilin Yuan,
Liang Ren,
Yuzhang Wu,
Yongwen Chen

Affiliations

Zeqing Feng: Institute of Immunology, PLA, Third Military Medical University, Chongqing, 400038, People’s Republic of China
Bo Diao: Department of Medical Laboratory Center, General Hospital of Central Theater Command, Wuhan, Hubei province, 430015, People’s Republic of China
Rongshuai Wang: Hubei Chongxin Judicial Expertise Center, Wuhan, Hubei province, 430415, People’s Republic of China
Gang Wang: Department of Medical Laboratory Center, General Hospital of Central Theater Command, Wuhan, Hubei province, 430015, People’s Republic of China
Chenhui Wang: Institute of Immunology, PLA, Third Military Medical University, Chongqing, 400038, People’s Republic of China
Yingjun Tan: Department of Medical Laboratory Center, General Hospital of Central Theater Command, Wuhan, Hubei province, 430015, People’s Republic of China
Liang Liu: Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan, Hubei province, 430030, People’s Republic of China
Changsong Wang: Department of Pathology, 989th Hospital of PLA, Luoyang, 471000, Henan Province, People’s Republic of China
Ying Liu: Department of Medical Laboratory Center, General Hospital of Central Theater Command, Wuhan, Hubei province, 430015, People’s Republic of China
Yueping Liu: Department of Medical Laboratory Center, General Hospital of Central Theater Command, Wuhan, Hubei province, 430015, People’s Republic of China
Zilin Yuan: Department of Medical Laboratory Center, General Hospital of Central Theater Command, Wuhan, Hubei province, 430015, People’s Republic of China
Liang Ren: Hubei Chongxin Judicial Expertise Center, Wuhan, Hubei province, 430415, People’s Republic of China
Yuzhang Wu: Institute of Immunology, PLA, Third Military Medical University, Chongqing, 400038, People’s Republic of China
Yongwen Chen: Institute of Immunology, PLA, Third Military Medical University, Chongqing, 400038, People’s Republic of China

DOI: https://doi.org/10.21769/BioProtoc.5002
Journal volume & issue: Vol. 10, no. 7

Abstract

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While lymphocytopenia is a common characteristic of patients infected by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the mechanisms responsible for this depletion are unclear. With the tissue samples of the spleens and lymph nodes (LNs) from six cases, immunohistochemistry demonstrated ACE2 (angiotensin-converting enzyme 2), the potential receptor of SARS-CoV-2, expresses on tissue-resident CD169+ macrophages in spleens and LNs.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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