Nature Communications (Mar 2020)

Trajectory-based differential expression analysis for single-cell sequencing data

  • Koen Van den Berge,
  • Hector Roux de Bézieux,
  • Kelly Street,
  • Wouter Saelens,
  • Robrecht Cannoodt,
  • Yvan Saeys,
  • Sandrine Dudoit,
  • Lieven Clement

DOI
https://doi.org/10.1038/s41467-020-14766-3
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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Downstream of trajectory inference for cell lineages based on scRNA-seq data, differential expression analysis yields insight into biological processes. Here, Van den Berge et al. develop tradeSeq, a framework for the inference of within and between-lineage differential expression, based on negative binomial generalized additive models.