PLoS ONE (Jan 2020)

Biological activities of a recombinant fortilin from Fenneropenaeus merguiensis.

  • Ureporn Kedjarune-Leggat,
  • Uraipan Saetan,
  • Anchana Khongsaengkaeo,
  • Sudarat Suwannarat,
  • Panchalika Deachamag,
  • Monwadee Wonglapsuwan,
  • Rawiwan Pornprasit,
  • Wanwisa Thongkamwitoon,
  • Parujee Phumklai,
  • Jirapan Chaichanan,
  • Wilaiwan Chotigeat

DOI
https://doi.org/10.1371/journal.pone.0239672
Journal volume & issue
Vol. 15, no. 10
p. e0239672

Abstract

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Human Fortilin, an antiapoptotic protein, has also been implicated in several diseases; however, several potential uses of fortilin have also been proposed. Bearing the implications of fortilin in mind, fortilin analog, which has no complication with diseases, is required. Since a recombinant full-length fortilin from Fenneropenaeus merguiensis (rFm-Fortilin (FL)) reported only 44% (3e-27) homologous to human fortilin, therefore the biological activities of the Fm-Fortilin (FL) and its fragments (F2, F12, and F23) were investigated for potential use against HEMA toxicity from filling cement to pulp cell. The rFm-Fortilin FL, F2, 12, and F23 were expressed and assayed for proliferation activity. The rFm-Fortilin (FL) showed proliferation activity on human dental pulp cells (HDPCs) and protected the cells from 2-hydroxy-ethyl methacrylate (HEMA) at 1-20 ng/ml. In contrast, none of the rFm-Fortilin fragments promoted HDPC growth that may be due to a lack of three conserved amino acid residues together for binding with the surface of Rab GTPase for proliferative activity. In addition, rFm-Fortilin (FL) activated mineralization and trend to suppressed production of proinflammatory cytokines, including histamine (at 10 ng/ml) and TNF-α (at 100 ng/ml). Besides, the rFm-Fortilin (FL) did not mutate the Chinese hamster ovary (CHO) cell. Therefore, the rFm-Fortilin (FL) has the potential use as a supplementary medical material to promote cell proliferation in patients suffering severe tooth decay and other conditions.