Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model

Abstract

Abstract Background The difficulty of achieving targeted drug delivery following administration of presently marketed anticancer therapeutics is still a concern. Metallic nanoparticles (NPs) appear to be promising in this regard. The present study focused on the use of gold nanoparticles (AuNPs) as a drug carrier for anticancer Doxorubicin (DOX) forming DOX–AuNPs nanocomposite. The anticancer effect of the prepared nanocomposite was evaluated using SRP essay on breast cancer cell line (MCF7) for different incubation times (24 h,48, and72hr). The prepared DOX–AuNPs nanocomposite was investigated by UV–visible spectroscopy, TEM, fluorescence spectroscopy, and FTIR spectroscopy. Results Our results showed that the prepared AuNPs and DOX–AuNPs nanocomposite have spherical and small size10 ± 2 nm and 12 ± 2 nm respectively. The potential cytotoxicity of the DOX-AuNPs nanocomposite on the MCF7 cell line was significantly increased compared to free DOX. The 20 µM DOX- AuNPs nanocomposite produced a similar decrease in cell survival as 80 µM free DOX. Conclusion Future work is in progress to investigate the positive effects of the prepared nanocomposite for chemo-photothermal combination treatment.

Keywords

• Gold nanoparticles
• Cancer
• Drug delivery
• Doxorubicin
• Breast carcinoma cell line (MCF7)