Protective Role of Pomegranate Juice and its Peel Extract on Steroid Induced Raised Serum Creatinine in Mice

Abstract

Objective: To determine the protective role of Pomegranate Juice (PJ) and Pomegranate Peel Extract (PPE) on steroid induced serum creatinine level in mice. Study Design: Randomized control trial. Place and Duration of Study: Department of Anatomy, Army Medical College Rawalpindi, in collaboration with the National Institute of Health (NIH), Islamabad, from May 1st to June 30th , 2015. Materials and Methods: Forty healthy mice (BALB/c strain), weighing 25-30 gms on average were randomly divided into four groups, each group having five male and female mice. Group A was control group and groups B, C and D were experimental groups. Mice in experimental groups were injected Nandrolone decanoate, 1 mg/100 gm body weight, intramuscularly in the hind limb once a week for 8 weeks. Mice in experimental group C were also given pomegranate Juice daily, 3ml/kg body weight by oral gavage tube for 8 weeks. Mice in experimental group D were given Pomegranate Peel Extract daily, 200mg/kg bodyweight through oral gavage tube for 8 weeks. After eight weeks 5ml blood was drawn from mice through cardiac puncture just before the sacrifice of animals. Data were entered and analyzed by using SPSS version 21. ANOVA test was applied for intergroup comparison of quantitative variables. P-value of <0.05 was taken as significant. Results: Serum creatinine level was significantly higher in experimental group B. It was improved statistically when compared with pomegranate juice and pomegranate peel extract administered experimental groups C and D, respectively. When results of experimental groups C and D were compared with each other no statistical significance was present. Conclusion: Both forms of pomegranate equally ameliorate the steroid induced effects on serum creatinine level in mice.

Keywords

• androgenic anabolic steroids, pomegranate juice, pomegranate peel extract, serum creatinine level