Jichu yixue yu linchuang (Dec 2022)

IL-6 trans-signaling inhibitor sgp130Fc suppresses inflammation and regulates immune cells in mice with sepsis

  • SHI Dan-dan, JIANG Su-fang, WANG Qian, ZHAO Peng-bo, LIU Ya

DOI
https://doi.org/10.16352/j.issn.1001-6325.2022.12.1895
Journal volume & issue
Vol. 42, no. 12
pp. 1895 – 1899

Abstract

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Objective To investigate effects of IL-6 trans-signaling on immuno-inflammatory regulation in cecum ligation and puncture (CLP)-induced mice with sepsis. Methods Eighty mice were divided randomly into four groups: sham group (n=15), CLP group (n=30), CLP+sgp130Fc group (n=20) and sgp130Fc group (n=15). Sgp130Fc 0.5 mg/kg was injected intraperitoneally one hour after CLP in the CLP+sgp130Fc group and sgp130Fc group. Body weight and survival rate of mice were monitored daily during the research period (10 days). At 24 hrs after CLP, IL-6,IL-10, MCP-1 and TNFα were detected by ELISA; Peripheral blood leukocytes were separated and lymphocyte subgroups were sorted with the flow cytometry. Results Compared with sham group, the survival rate and body weight decreased significantly(P＜0.05). At 24 hrs after CLP, the expression of IL-6, IL-10, MCP-1 and TNFα increased significantly(P＜0.05). Lower level of the CD3+ CD4+ T cell and CD45+ CD19+ B cell but higher level of the CD3+ CD8+ T cell in the CLP group were found (P＜0.05). Compared with the CLP group, the survival rate was increased significantly(P＜0.05). At 24 hrs after CLP, the expression of IL-6, IL-10, MCP-1 and TNFα decreased significantly, and higher level of CD3+ CD4+ T cell and CD45+ CD19+ B cell but lower level of CD3+ CD8+ T cell in the CLP+ sgp130Fc group(P＜0.05) were recorded. Conclusions Inhibition of IL-6 trans-signaling can reduce mortality of mice with sepsis, which is potentially related to the regulation of early peripheral blood lymphocyte subgroups and inhibition of inflammatory responses.

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