Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation

Tobias Raisch; Chung-Te Chang; Yevgen Levdansky; Sowndarya Muthukumar; Stefan Raunser; Eugene Valkov

doi:10.1038/s41467-019-11094-z

Nature Communications (Jul 2019)

Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation

Tobias Raisch,
Chung-Te Chang,
Yevgen Levdansky,
Sowndarya Muthukumar,
Stefan Raunser,
Eugene Valkov

Affiliations

Tobias Raisch: Department of Biochemistry, Max Planck Institute for Developmental Biology
Chung-Te Chang: Department of Biochemistry, Max Planck Institute for Developmental Biology
Yevgen Levdansky: Department of Biochemistry, Max Planck Institute for Developmental Biology
Sowndarya Muthukumar: Department of Biochemistry, Max Planck Institute for Developmental Biology
Stefan Raunser: Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology
Eugene Valkov: Department of Biochemistry, Max Planck Institute for Developmental Biology

DOI: https://doi.org/10.1038/s41467-019-11094-z
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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The CCR4-NOT complex shortens poly(A) tails of messenger RNAs. By biochemical reconstitution of the entire human CCR4-NOT complex, the authors show the stimulatory roles of non-enzymatic subunits and the importance of the interaction between CAF40 and RNA binding proteins in targeted deadenylation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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