Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

Abstract

Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was generated by polyethylene glycol (PEG) 6000 and mannitol, related to drought stress and growth of Waha durum wheat genotype. Two tests were carried out in a growth chamber; the first comprises seed germination into Petri dishes in the presence of different concentrations of the two osmoticums (0, 5, 10, 15 and 20 % of PEG6000 and mannitol). The second test was carried out in nutrient solution BD medium. Our results shows that Both PEG -6000 and mannitol reduced germination. Therefore, a rapid increase was observed in the rate of germination both for the control plants and the plants subjected to a concentration of 5 g/L and 10 g/L and changes in proportion to the time. For the concentration of 15 g/L and 20 g/L, this phase is very short, which explains the reduced germination rate due to the inhibitory effect of the two osmoticums on germination. In this study, PEG-6000 treatments resulted in an increase of some proteins and a decrease of others. Waha displayed 12 bands for control plants, 40 bands for PEG-6000 stressed plants (all treatments) and 35 bands for mannitol treatments.

Keywords

• germination
• mannitol
• peg6000
• sds page
• wheat