Exposure to polystyrene microplastic beads causes sex-specific toxic effects in the model insect Drosophila melanogaster

Abstract

Abstract The toxicity of MPs on aquatic creatures has been extensively studied, but little attention was paid to terrestrial organisms. To fill this gab, we conducted a series of experiments using Drosophila as a model organism to understand whether exposure to different concentrations (0.005, 0.05, 0.5 µg/ml) of polystyrene microplastics (PS-MPs) beads (2 µm in size) can impact flies feeding activity, digestion and excretion. The ability of flies to distinguish between normal and PS-MPs treated food media was tested first, and then we evaluated the effects of a 7-day short-term exposure to PS-MPs on food intake, mortality, starvation resistance, fecal pellet count, and the cellular structure of mid gut cells. The results revealed that flies can really differentiate and ignore MPs-treated food. We discovered sex-specific effects, with male flies being more sensitive to PS-MPs, with all males dying after 14 days when exposed to 0.5 µg/ml of PS-MPs, whereas female flies survived more. All male flies exposed to PS-MPs died after 24 h of starvation. Midgut cells showed concentration-dependent necrosis and apoptosis in response to PS-MPs. Our findings provide new insights into MPs toxicity on terrestrial organisms and giving a warning that management measures against MPs emission must be taken.