Nature Communications (Apr 2021)
Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs
- Majid Haddad Momeni,
- Folmer Fredslund,
- Bastien Bissaro,
- Olanrewaju Raji,
- Thu V. Vuong,
- Sebastian Meier,
- Tine Sofie Nielsen,
- Vincent Lombard,
- Bruno Guigliarelli,
- Frédéric Biaso,
- Mireille Haon,
- Sacha Grisel,
- Bernard Henrissat,
- Ditte Hededam Welner,
- Emma R. Master,
- Jean-Guy Berrin,
- Maher Abou Hachem
Affiliations
- Majid Haddad Momeni
- Department of Biotechnology and Biomedicine, Technical University of Denmark
- Folmer Fredslund
- The Novo Nordisk Center for Biosustainability
- Bastien Bissaro
- INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF)
- Olanrewaju Raji
- Department of Chemical Engineering and Applied Chemistry, University of Toronto
- Thu V. Vuong
- Department of Chemical Engineering and Applied Chemistry, University of Toronto
- Sebastian Meier
- Department of Chemistry, Technical University of Denmark
- Tine Sofie Nielsen
- Department of Biotechnology and Biomedicine, Technical University of Denmark
- Vincent Lombard
- Architecture et Fonction des Macromolécules Biologiques, UMR 7257 CNRS, USC 1408, Aix Marseille Univ
- Bruno Guigliarelli
- Aix-Marseille Univ, CNRS, UMR7281 Unité de Bioénergétique et Ingénierie des Protéines (BIP)
- Frédéric Biaso
- Aix-Marseille Univ, CNRS, UMR7281 Unité de Bioénergétique et Ingénierie des Protéines (BIP)
- Mireille Haon
- INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF)
- Sacha Grisel
- INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF)
- Bernard Henrissat
- Architecture et Fonction des Macromolécules Biologiques, UMR 7257 CNRS, USC 1408, Aix Marseille Univ
- Ditte Hededam Welner
- The Novo Nordisk Center for Biosustainability
- Emma R. Master
- Department of Chemical Engineering and Applied Chemistry, University of Toronto
- Jean-Guy Berrin
- INRAE, Aix Marseille Univ, Biodiversité et Biotechnologie Fongiques (BBF)
- Maher Abou Hachem
- Department of Biotechnology and Biomedicine, Technical University of Denmark
- DOI
- https://doi.org/10.1038/s41467-021-22372-0
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 13
Abstract
Microbial oxidoreductases are key in biomass breakdown. Here, the authors expand the specificity and redox scope within fungal auxiliary activity 7 family (AA7) enzymes and show that AA7 oligosaccharide dehydrogenases can directly fuel cellulose degradation by lytic polysaccharide monooxygenases.
