Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway

Mu-Kuan Chen; Yen-Tze Liu; Jen-Tsun Lin; Chia-Chieh Lin; Yi-Ching Chuang; Yu-Sheng Lo; Yi-Ting Hsi; Ming-Ju Hsieh

Biomedicine & Pharmacotherapy (Sep 2019)

Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway

Mu-Kuan Chen,
Yen-Tze Liu,
Jen-Tsun Lin,
Chia-Chieh Lin,
Yi-Ching Chuang,
Yu-Sheng Lo,
Yi-Ting Hsi,
Ming-Ju Hsieh

Affiliations

Mu-Kuan Chen: Department of Otorhinolaryngology, Head and Neck Surgery, Changhua Christian Hospital, Changhua 500, Taiwan
Yen-Tze Liu: Department of Family Medicine, Changhua Christian Hospital, Changhua 500, Taiwan; Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Department of Holistic Wellness, Mingdao University, Changhua 52345, Taiwan
Jen-Tsun Lin: Division of Hematology and Oncology, Department of Medicine, Changhua Christian Hospital, Changhua 500, Taiwan; School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan
Chia-Chieh Lin: Oral Cancer Research Center, Changhua Christian Hospital, Changhua 500, Taiwan
Yi-Ching Chuang: Oral Cancer Research Center, Changhua Christian Hospital, Changhua 500, Taiwan
Yu-Sheng Lo: Oral Cancer Research Center, Changhua Christian Hospital, Changhua 500, Taiwan
Yi-Ting Hsi: Oral Cancer Research Center, Changhua Christian Hospital, Changhua 500, Taiwan
Ming-Ju Hsieh: Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan; Department of Holistic Wellness, Mingdao University, Changhua 52345, Taiwan; Oral Cancer Research Center, Changhua Christian Hospital, Changhua 500, Taiwan; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan; Corresponding author at: Oral Cancer Research Center, Changhua Christian Hospital, 500 South School Street, No. 135, Changhua, Taiwan.

Journal volume & issue: Vol. 117

Abstract

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Background: Pinosylvin possesses several biological properties, including anti-inflammatory, antitumor, and antioxidant characteristics. However, the effects of pinosylvin on the migration and invasion of human oral cancer cells and the underlying mechanisms remain unclear. Hypothesis/purpose: In this research, we investigated the outcome of different concentrations of pinosylvin (0–80 μM) on the metastatic and invasive abilities of SAS, SCC-9, and HSC-3 cells. Methods and results: Western blotting assay and Gelatin zymography assay indicated that pinosylvin inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and reduced its protein level but increased the expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). Additionally, the wound healing assay and Transwell method showed that pinosylvin reduced the migration of SAS, SCC-9 and HSC-3 oral cancer cells. Besides, pinosylvin decreased the phosphorylation of ERK1/2 protein experssion in both SAS and SCC-9 cells. Conclusion: These results indicate that pinosylvin is a potential anticancer agent for preventing oral cancer metastasis.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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